aim; goal; purpose
look; eyes; expression of eyes
「目差し」 has another reading with different meanings.
(a) look; gaze
to aim at (for, to do, to become); to try for; to have an eye on
高い目差しを持って努力した。
I worked hard with high aspirations.
彼の目差しは世界一のシェフになることだ。
His goal is to become the world's best chef.
チーム全員が同じ目差しで練習に取り組んだ。
The whole team worked on practice with the same goal.